An Efficient Method for Adventitious Root Induction from Stem Segments of Brassica Species

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An Efficient Method for Adventitious Root Induction from Stem Segments of Brassica Species

Plant propagation via in vitro culture is a very laborious and time-consuming process. The growth cycle of some of the crop species is slow even in the field and the consistent commercial production is hard to maintain. Enhanced methods of reduced cost, materials and labor significantly impact the research and commercial production of field crops. In our studies, stem-segment explants of Brassi...

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Biochemical Basis of Adventitious Root Formation on Etiolated Stem Segments

Segments (2.5-cm-long) of Populus nigra L. obtained from etiolated axillary branches did not root in water or auxin alone, but rooted in 0.5% ribose, glucose and sucrose and more profusely with 0.1 mg/1 in dole-acetic acid (IAA) or in dolebutyric acid (IBA) added to the medium. 5-fluorodeoxyuridine (FUDR), 5-fluorouracil (FU), actinomycin-D and cycloheximide inhibited rooting. The deoxyribonucl...

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Analysis of indole-3-butyric acid-induced adventitious root formation on Arabidopsis stem segments.

Root induction by auxins is still not well understood at the molecular level. In this study a system has been devised which distinguishes between the two active auxins indole-3-butyric acid (IBA) and indole-3-acetic acid (IAA). IBA, but not IAA, efficiently induced adventitious rooting in Arabidopsis stem segments at a concentration of 10 microM. In wild-type plants, roots formed exclusively ou...

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ژورنال

عنوان ژورنال: Frontiers in Plant Science

سال: 2016

ISSN: 1664-462X

DOI: 10.3389/fpls.2016.00943